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An inexpensive and compact underwater digital camera imaging system was developed to collect in situ high resolution images of flocculated suspended sediment at depths of up to 60 meters. The camera has a field of view of 3.7 × 2.8 mm and can resolve particles down to 5 . Depending on the degree of flocculation, the system is capable of accurately sizing particles to concentrations up to 500 mg/L. The system is fast enough to allow for profiling whereby size distributions of suspended particles and flocs can be provided at multiple verticals within the water column over a relatively short amount of time (approximately 15 min for a profile of 15 m). Using output from image processing routines, methods are introduced to estimate the mass suspended sediment concentration (SSC) from the images and to separate identified particles into sand and mud floc populations. The combination of these two methods allows for the size and concentration estimates of each fraction independently. The camera and image analysis methods are used in both the laboratory and the Mississippi River for development and testing. Output from both settings are presented in this study.